分子印迹技术的特点

1.预定性，即它可以根据不同的目的制备不同的MIPs，以满足各种不同的需要。

2.识别性，即MIPS是按照模板分子定做的，可专一地识别印迹分子。

3.实用性，即它可以与天然的生物分子识别系统如酶与底物、抗原与抗体、受体与激素相比拟，但由于它是由化学合成的方法制备的，因此又有天然分子识别系统所不具备的抗恶劣环境的能力，从而表现出高度的稳定性和长的使用寿命。

目前,根据模板分子和聚合物单体之间形成多重作用点方式的不同，分子印迹技术可以分为两类：

1.共价键法（预组装方式）

聚合前印迹分子与功能单体反应形成硼酸酷、西夫碱、亚胺、缩醛等衍生物，通过交联剂聚合产生高分子聚合物，用水解等方法除去印迹分子即得到共价结合型分子印迹聚合物。

2.非共价键法（自组装方式）

非共价键法是制备分子印迹聚合物最有效且最常用的方法。这些非共价键包括静电引力（离子交换）、氢键、金属鳌合、电荷转移、疏水作用以及范德华力等。其中最重要的类型是离子作用，其次是氢键作用。

Objective: to use electricity spinning method for preparation of e. coli phage, molecularly imprinted polymer membranes of the template. In this test requirements with the whole process of scientific research, including data collection, the subject designing, experimental operation and professional writing the thesis. While improving students' scientific thinking ability and practical ability.

Methods: using mpeg precipitation + chloroform extraction smoke concentration purification phage reach 1012 / ml, concentration of PVA by adjusting voltage, high flow rate, and spinning, the preparation conditions after film by scanning electron microscope (SEM) spinning solution concentration, flow, voltage, high on spinning fiber diameter and the morphology of the influence. Using the electrostatic spinning technology in preparation for the conditions for the "shell" with PVA, escherichia coli phage as "core" of molecularly imprinted membrane, and through the double plaque method of observing AGAR bite after encapsulation testing of bioactive phage.

Results: the successful condensed phage reach 1012 / ml. In the process of preparation conditions with water groping for solvent for 7% PVA concentration (w/w) of membrane 2, Before joining ethanol and SM, spinning is unstable, lots of broken wire and beads, liquid membrane, not because of time, not continue, experiment which terminates. Turn on the morphology, PVA spinning fiber diameter with PVA concentration, the relationship between voltage

Conclusions: (1) by the preparation of several membranes observed results show that, with the increase of spinning solution concentration, fiber diameter increase gradually, the distribution of fiber diameter with, With the increase of fiber diameter, voltage did not change significantly.

2, the experiment of polymer matrix phage and spinning compatibility of PVA were evaluated.

[key] : a phage maximizing electrostatic spinning PVA

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