高手帮忙把摘要翻译成英文,谢谢了!

高手帮忙把摘要翻译成英文,谢谢了!,第1张

Objective: to use electricity spinning method for preparation of e. coli phage, molecularly imprinted polymer membranes of the template. In this test requirements with the whole process of scientific research, including data collection, the subject designing, experimental operation and professional writing the thesis. While improving students' scientific thinking ability and practical ability.

Methods: using mpeg precipitation + chloroform extraction smoke concentration purification phage reach 1012 / ml, concentration of PVA by adjusting voltage, high flow rate, and spinning, the preparation conditions after film by scanning electron microscope (SEM) spinning solution concentration, flow, voltage, high on spinning fiber diameter and the morphology of the influence. Using the electrostatic spinning technology in preparation for the conditions for the "shell" with PVA, escherichia coli phage as "core" of molecularly imprinted membrane, and through the double plaque method of observing AGAR bite after encapsulation testing of bioactive phage.

Results: the successful condensed phage reach 1012 / ml. In the process of preparation conditions with water groping for solvent for 7% PVA concentration (w/w) of membrane 2, Before joining ethanol and SM, spinning is unstable, lots of broken wire and beads, liquid membrane, not because of time, not continue, experiment which terminates. Turn on the morphology, PVA spinning fiber diameter with PVA concentration, the relationship between voltage

Conclusions: (1) by the preparation of several membranes observed results show that, with the increase of spinning solution concentration, fiber diameter increase gradually, the distribution of fiber diameter with, With the increase of fiber diameter, voltage did not change significantly.

2, the experiment of polymer matrix phage and spinning compatibility of PVA were evaluated.

[key] : a phage maximizing electrostatic spinning PVA

SEM的应用范围

生物:种子、花粉、细菌医学:血球、病毒动物:大肠、绒毛、细胞、纤维>材料:陶瓷、高分子粉末、环氧脂化学、物理、地质、冶金、矿物、污泥(杆菌)、机械、电机、及导电性样品如半导体(IC、线宽测量、断面、结构观察)电子材料等。

SEM的优点具有较光学显微镜好的解析度具有较大的景深,利于观察样品表面形态和尺度。能提供具实体感的立体影像。

进行元素分析的信号主要是作用区发射的元素特征X射线,俄歇电子,

阴极荧光,背散射电子等,特征能量损失电子。

SEM

常常使用:EDS,WDS

分光特征X射线;

俄歇电子需要超高真空环境,往往以俄歇电子谱仪的商品出现,也可归入扫描电镜范畴;背散射电子携带微区不同平均原子序数区域的反差信息,定性的了解原子序数高低或者材料密度分布;阴极荧光光谱范围在紫外,可见或红外波段所发射的电磁辐射,这种现象可以被用来检测矿物,半导体和生物样品中痕量元素(ppm级别,用X射线波谱WDS或者X射线能谱EDS都是不能实现的)的分布。

TEM常使用特征x射线和特征能量损失电子,


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